Detection of antigen-receptor gene rearrangement clonality for diagnosis of lymphoma.
- Author:
Xiao-Bo LI
1
;
Yin-Ping WANG
;
Jing-Na GAO
;
Chao WANG
;
Ya-Bin ZOU
;
Hai-Ying WANG
;
Yu-Lai ZHOU
;
Xiu-Mei DUAN
Author Information
1. Center of Pathological Diagnosis, Jinlin University, Changchun, Jilin Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Child;
Female;
Gene Rearrangement, T-Lymphocyte;
Humans;
Lymphoma;
diagnosis;
pathology;
Lymphoma, B-Cell;
diagnosis;
pathology;
Lymphoma, T-Cell;
diagnosis;
pathology;
Male;
Middle Aged;
Receptors, Antigen, T-Cell;
genetics;
Young Adult
- From:
Journal of Experimental Hematology
2012;20(4):906-911
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the significance of detecting the antigen-receptor gene rearrangement clonality in the diagnosis of lymphoma. Paraffin-embedding and HE staining of samples from 31 patients with lymphomas were performed for morphologic observation by light microscope. Immunophenotype was analyzed by the immunohistochemistry (IHC) method. The clonality of antigen-receptor gene rearrangement was detected by BIOMED-2 Assay Kit. The results showed that among the 31 cases, 12 cases were suspected to be T-cell lymphoma, 1 case was suspected to be T-cell reactive hyperplasia, and 16 cases were suspected to be B-cell lymphoma, 2 cases were B-cell reactive hyperplasia. The detection results showed that the positivity of Ig gene rearrangement clonality was 94.44% (17/18), the positivity of TCR gene rearrangement clonality was 92.31% (12/13), the other two cases were negative. Finally, 12 cases were diagnosed to be T-cell lymphoma and 17 cases were B-cell lymphoma. The other two cases were reactive lymphoid proliferations. And the positivity rate in the 31 patients with lymphomas was 93%. It is concluded that the detection of antigen-receptor gene rearrangement clonality is a useful assistant method in the diagnosis of lymphoma.
