Effect of DAPT on proliferation and apoptosis of human multiple myeloma cell line RPMI8226.
- Author:
Ying-Ying YUAN
1
;
Zhi-Yong ZENG
;
Jun-Min CHEN
Author Information
1. The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Basic Helix-Loop-Helix Transcription Factors;
metabolism;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Dipeptides;
pharmacology;
Homeodomain Proteins;
metabolism;
Humans;
Multiple Myeloma;
metabolism;
pathology;
Receptor, Notch1;
metabolism;
Transcription Factor HES-1
- From:
Journal of Experimental Hematology
2012;20(4):922-925
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to explore the effect of DAPT (N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycinet-butyl ester) on proliferation in vitro of human multiple myeloma cell line RPMI8226 and its underlying mechanism. The proliferation of RPMI8226 cells was detected by CCK-8 method; flow cytometry was employed to assay the cell apoptosis rate;the expressions of Notch1 and Hes1 proteins were detected by Western blot. The results indicated that the proliferation of human RPMI8226 cells significantly decreased after treatment with DAPT 0.5 - 5.0 µmol/L for 24 - 72 h (P < 0.05) in a concentration- and time-dependent manner. DAPT significantly induced apoptosis of RPMI8226 cells (P < 0.05). The expressions of Notch1 and Hes1 proteins were gradually downregulated with the increase of DAPT concentration. It is concluded that the DAPT can inhibit the proliferation of RPMI8226 cells, which may be related with the down-regulation of the protein expression of Notchl and Hes1.
