Detection of tissue factor-positive microparticles and its clinical significance in the haemostatic disorder.
- Author:
Li-Li ZHOU
1
;
Yue HAN
;
Qian ZHU
;
Lu-Ping HU
;
Shi-Xiang ZHAO
;
Ming-Qing ZHU
;
Lan DAI
;
Wen-Hong SHEN
;
Li CHEN
;
De-Pei WU
Author Information
1. Department of Hematology, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Blood Coagulation Disorders;
blood;
Case-Control Studies;
Female;
Flow Cytometry;
Humans;
Leukemia, Promyelocytic, Acute;
blood;
Male;
Middle Aged;
Prognosis;
Thromboplastin;
metabolism;
Young Adult
- From:
Journal of Experimental Hematology
2012;20(4):937-940
- CountryChina
- Language:Chinese
-
Abstract:
Objective of this study was to detect the level of tissue factor-positive microparticles (TF(+)MP) by flow cytometry (FCM) and to analyze its clinical significance in the haemostatic disorder. TF(+) MP was detected by FCM using antibody CD142-PE in 25 cases of acute promyelocytic leukemia (APL), 20 cases of hemostatic diseases and 20 healthy adults as controls. The differences of TF(+) MP between various groups were determined. The results showed that the level of TF(+) MP in the patients with thrombotic complications was significantly higher than that in the healthy adults (P < 0.05). The TF(+) MP level was higher in the patient with APL than that in the healthy adults, especially in course before therapy (P < 0.01), but the difference was not statistically significant in the patient with APL after therapy and the healthy adults. Among these patient with APL, the level of TF(+) MP in the 18 patients who complicated with disseminated intravascular coagulation (DIC) was also higher than that in the healthy adults (P < 0.05), but the level of TF(+) MP in the other 7 patients who did not complicate with DIC was similar before and after treatment. It is concluded that the method of TF(+) MP detection by FCM is feasible and simple, it is useful for the diagnosis of thrombotic disorder, and helps evaluation for the prognosis of APL patient.
