Identification of a novel HLA allele, HLA-B*35:03:07, by sequencing-based typing.
- Author:
Pei-Cong ZHAI
1
;
Yan LIU
;
Yi ZHANG
;
Xiang-Min NIE
;
Yong-Hong SONG
;
Yun-Long ZHUANG
;
Chuan-Fu ZHU
Author Information
1. HLA Laboratory, Blood Center of Shandong Province, Jinan, Shandong Province, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
Asian Continental Ancestry Group;
genetics;
HLA-B Antigens;
genetics;
Humans;
Male;
Sequence Analysis, DNA
- From:
Journal of Experimental Hematology
2012;20(4):1010-1013
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to analyze and identify a novel HLA allele in Chinese population. A new HLA-B allele which is closely related to HLA-B*35:03:01 was initially detected by PCR-SSOP, then DNA sequencing was performed to identify the difference between the novel allele and HLA-B*35:03:01 allele. The result showed that the sequence of the new allele was different from all other known sequence. It differs from the closest matching HLA-B*35:03:01 by a single substitution at position 387 C→G in exon 3, no resulting in amino acid change. It is concluded that this allele is a novel one and has been officially named B*35:03:07 by the WHO Nomenclature Committee.
