Expression of c-MPL in leukemic stem cells from acute myeloid leukemia patients.
- Author:
Pei YU
1
;
Shao-Wei QIU
;
Qing RAO
;
Dong LIN
;
Hai-Yan XING
;
Ke-Jing TANG
;
Zheng TIAN
;
Min WANG
;
Jian-Xiang WANG
Author Information
1. Chinese Academy of Medical Sciences, Tianjin, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Case-Control Studies;
Child;
Female;
Humans;
Leukemia, Myeloid, Acute;
metabolism;
pathology;
Male;
Middle Aged;
Neoplastic Stem Cells;
metabolism;
Receptors, Thrombopoietin;
metabolism;
Young Adult
- From:
Journal of Experimental Hematology
2012;20(5):1052-1055
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the expression of c-MPL in acute myeloid leukemia (AML) and the correlation of the c-MPL expression with CD34 and CD38, so as to define the expression of c-MPL in leukemic stem cells. The expression levels of CD34, CD38 and c-MPL were detected by flow cytometry in bone marrow cells from 29 newly diagnosed AML patients. The relationship of c-MPL positive cell ratio with clinical parameters and correlation of c-MPL with CD34 and CD38 expression in AML patients were analyzed. The results showed that expression level of c-MPL in AML patients was significantly higher than that of normal controls (P < 0.05), and the expression level of c-MPL did not correlate with age, sex, white blood cell count, AML1-ETO fusion gene and remission after chemotherapy, but the expression of c-MPL in M2 and M5 patients was higher than that of normal control (P < 0.05). Expression of c-MPL in CD34 positive AML patients was obviously higher than that in CD34 negative AML patients (P < 0.01). c-MPL was significantly higher expressed in CD34(+) cells than that in CD34(-) cells (P < 0.001), while c-MPL expression was not significantly different between CD34(+)CD38(-) and CD34(+)CD38(-) cell groups. Positive correlation between c-MPL and CD34 expression was observed (r = 0.380, P = 0.042). It is concluded that expression of c-MPL is higher in AML patients, and positively correlates with the expression level of CD34. The c-MPL expresses in leukemic stem cells.
