Effect of mPGES-1 inhibitor MK886 on cell cycle of leukemia HL-60 cells.
- Author:
Yi-Qing LI
1
;
Song-Mei YIN
;
Shuang-Feng XIE
;
Xiu-Ju WANG
;
Li-Ping MA
;
Da-Nian NIE
;
Yu-Dan WU
Author Information
1. Department of Internal Hematology, SUN Yat-Sen University, Guangzhou, China.
- Publication Type:Journal Article
- MeSH:
Cell Cycle;
drug effects;
HL-60 Cells;
Humans;
Indoles;
pharmacology;
Intramolecular Oxidoreductases;
antagonists & inhibitors;
Leukemia;
metabolism;
pathology;
Prostaglandin-E Synthases
- From:
Journal of Experimental Hematology
2012;20(5):1072-1076
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of a microsomal prostaglandin E synthase-1 (mPGES-1) inhibitor MK886 on cell cycle of the human acute myeloid leukemia HL-60 cells. HL-60 cells were treated with different concentration of MK886 (10, 25, 50 µmol/L) for 24 h. Flow cytometry, Western blot and ELISA were used to measure cell cycle, cyclin D1, mPGES-1, PGE(2), Akt, P-Akt and C-MYC. The results indicated that after treated with MK886, the percentage of HL-60 cells decreased in G(0)/G(1) phase and increased in S phase, and expressions of mPGES-1, cyclin D1, P-Akt and C-MYC and synthesis of PGE(2) decreased significantly. It is concluded that MK886 can arrest HL-60 cells in G(0)/G(1) phase, the mechanism of which is possibly associated to inhibition of mPGES-1 expression, reduction of PGE(2) synthesis, suppression of Akt phosphorylation and C-MYC expression, down-regulation of cyclin D1 expression.
