Establishment of a new method for screening of CBFB-MYH11 fusion gene in acute myeloid leukemia and its value in clinical use.
- Author:
Chao CHEN
1
;
Zhi-Peng LI
;
Quan-Yi LU
;
Zhi-Ming LIU
Author Information
1. Beijing Normal University, Guangdong Province, China.
- Publication Type:Journal Article
- MeSH:
Case-Control Studies;
Core Binding Factor beta Subunit;
analysis;
genetics;
HL-60 Cells;
Humans;
Leukemia, Myeloid, Acute;
diagnosis;
genetics;
Myosin Heavy Chains;
analysis;
genetics;
Oncogene Proteins, Fusion;
analysis;
genetics;
Real-Time Polymerase Chain Reaction
- From:
Journal of Experimental Hematology
2012;20(5):1077-1081
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to establish new method for detecting CBFB-MYH11 fusion gene in acute myeloid leukemia (AML) and to evaluate its value in clinical use. All fusion types of reported CBFB-MYH11 fusion gene were defined by search of references and databank, then the primers and probes were designed on this basis, and 3 positive plasmids and negative cell line as control were established. GUSB gene was also amplified as an internal reference. The primer/probe sets were tested with 3 positive plasmids and HL-60 cDNA using quantitative real-time PCR (qPCR) assays, which were then combined as a multiplex qPCR for simultaneous detection of CBFB-MYH11 and GUSB. After optimization, the multiplex qPCR assay demonstrated both high sensitivity (10 copies for all the 3 plasmids) and high specificity. Finally, the multiplex qPCR assay was clinically evaluated with 58 AML patients, and 4 CBFB-MYH11-positive cases (6.9%) were detected, involving A type (3 cases) and J type (1 case). By comparison, the multiplex qPCR assay showed results concordant with sequencing results, and detected one case that was missed by cytogenetic analysis. It is concluded that a novel qPCR method for screening of CBFB-MYH11 fusion gene in AML is established. This method is fast, comprehensive, sensitive, specific, reliable, and should consider to be a robust tool for identification and management of AML patients with CBFB-MYH11 fusion gene.
