Silencing of ICSBP/IRF8 expression in U226 cells and bone marrow mononuclear cells from patients with multiple myeloma.
- Author:
Gu JIA
1
;
Yan-Ping MA
;
Ling ZHANG
;
Yu-Jin LU
;
Hong LU
;
Ming-Xing CHANG
;
Hua-Ping ZHANG
Author Information
1. Department of Hematology, Shanxi Medical University Second Hospital, Taiyuan, Shanxi Province, China.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
metabolism;
Case-Control Studies;
Cell Line, Tumor;
DNA Methylation;
Gene Silencing;
Humans;
Interferon Regulatory Factors;
genetics;
metabolism;
Multiple Myeloma;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2012;20(5):1127-1130
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to investigate expression of interferon regulatory factors (ICSBP/IRF8) and the potential role of DNA methylation in silencing ICSBP/IRF8 gene in multiple myeloma (MM) cell line U266 and bone marrow mononuclear cells from 10 MM patients (MM-BMMNC). The bone marrow mononuclear cells from 10 healthy persons (N-BMMNC) were collected and used as normal controls. Expression of ICSBP/IRF8 gene was detected by real-time fluorescence quantitative PCR (using 2(-ΔΔCT) to calculate); DNA methylation level of the ICSBP/IRF8 gene was measured using methylation-specific PCR (using the ratio of interest gene ICSBP/IRF8 and internal reference β-actin expression as results). The results showed that as compared with N-BMMNC the lower expression of ICSBP/IRF8 gene was found in U266 cells and MM-BMMNC, the hypermethylation of the CpG island in the ICSBP/IRF8 promoter was observed, there were significant differences between N-BMMNC and MM-BMMNC or U266 cells (P < 0.05). It is concluded that the expression of ICSBP/IRF8 gene can be silenced in the MM-BMMNC and U226 cells. As the hypermethylation of CpG island in ICSBP/IRF8 promoter is a frequent event in MM cells, the ICSBP/IRF8 gene silencing caused by DNA methylation may take part in the pathogenesis and development of MM.
