Effects of interferon-γ on expression of adhesion molecules in human umbilical cord mesenchymal stromal cells.
- Author:
Rui-Yun QIAO
1
;
Hai BAI
;
Cun-Bang WANG
;
Jian-Feng OU
;
Hai-Ying ZHANG
;
Qiang ZHAO
Author Information
1. Department of Hematology, Lanzhou Military Area General Hospital, Lanzhou, Gansu Province, China.
- Publication Type:Journal Article
- MeSH:
Cell Adhesion Molecules;
metabolism;
Cells, Cultured;
Humans;
Interferon-gamma;
pharmacology;
Mesenchymal Stromal Cells;
cytology;
drug effects;
metabolism;
Umbilical Cord;
cytology
- From:
Journal of Experimental Hematology
2012;20(5):1195-1199
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the effects of interferon (IFN)-γ on expression of adhesion molecules in mesenchymal stromal cells derived from human umbilical cord tissue (UC-MSC). The UC-MSC were isolated from human umbilical cord by tissue culture. The expressions of specific markers on UC-MSC were detected by flow cytometry in the physiological condition. The adipogenic and osteogenic induction of UC-MSC was detected by alizarin and Oil red O staining. UC-MSC were exposed to IFN-γ (100, 1 000, 10 000 U/ml) for 24 h, the expressions of CD54, CD58, CD44, CD49d, CD62p, CD62L, CD102 and CD106 on cell surface were detected using flow cytometry. The results showed that in physiological condition, UC-MSC extremely low expressed CD102, CD106, CD62P, CD62L, while the expression of CD54 was relatively high (41.58 ± 0.83)%. When stimulated by IFN-γ, the expression of CD102, CD106, CD62P, CD62L increased slightly, but still low (< 5%), while CD54 and CD58 upregulated concentration-dependently up to (59.66 ± 1.36)% and (43.96 ± 0.62)% respectively. The expression of CD49d upregulated to (51.33 ± 0.74)% when UC-MSC exposed to IFN-γ 100 U/ml. CD44 increased to (73.22 ± 1.93)% when UC-MSC exposed to IFN-γ 1 000 U/ml. It is concluded that IFN-γ can elevate significantly the expression of CD54, CD49d, CD44 and CD58, but has no significant effect on CD102, CD106, CD62P and CD62L expression on the surface of UC-MSC.
