Quantitative PCR for early diagnosis of invasive fungal infections in patients with hematologic malignancies.
- Author:
Shu-Juan LIU
1
;
Xin WANG
;
Xiang-Hua WANG
;
Hai ZHOU
;
Dai YUAN
;
Hua JIANG
Author Information
1. Department of Hematology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Early Diagnosis;
Female;
Fungi;
Hematologic Neoplasms;
microbiology;
Humans;
Male;
Middle Aged;
Mycoses;
diagnosis;
Polymerase Chain Reaction;
methods;
Predictive Value of Tests;
Sensitivity and Specificity;
Young Adult
- From:
Journal of Experimental Hematology
2012;20(5):1225-1230
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish the approach of quantitative PCR (q-PCR) for diagnosis of invasive fungal infections (IFI) in patients with hematologic malignancies. Specimens from 40 patients with hematologic malignancies were chosen for q-PCR and galactomannan (GM) test. The 28S rRNA, a real high consensus sequence of fungi, was selected as target gene to design primer and probe. The DNA of fungal species was extracted from serum specimens. The results showed that q-PCR sensitivity, specificity, positive and negative predictive values were 0.89, 0.85, 0.89, 0.85 respectively; GM test sensitivity, specificity, positive and negative predictive values were 0.83, 0.80, 0.88, 0.73 respectively; as combined q-PCR with GM test, these values were 0.94, 0.85, 0.89, 0.92 respectively. It is concluded that the q-PCR assay can be used for early diagnosis for IFI in patients with hematologic malignancies, q-PCR combined with GM test can enhance the diagnosis sensitivity for IFI.
