Removal of αGal xenotransplantation antigen by a novel α-galactosidase.
- Author:
Hong-Wei GAO
1
;
Xue ZHANG
;
Su-Bo LI
;
Ying-Xia TAN
;
Guo-Qiang BAO
;
Ying-Li WANG
;
Li-Juan XU
;
Shou-Ping JI
;
Feng GONG
Author Information
1. Department of Blood Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, Heterophile;
immunology;
Cattle;
Dogs;
Epitopes;
Erythrocytes;
immunology;
Macaca mulatta;
Mice;
Mice, Inbred BALB C;
Rabbits;
Swine;
Transplantation, Heterologous;
alpha-Galactosidase;
immunology
- From:
Journal of Experimental Hematology
2012;20(5):1231-1234
- CountryChina
- Language:Chinese
-
Abstract:
αGal, a xenotransplantations antigen (XTA), can lead to hyper acute reaction (HAR) in xenotransplantation. α-Galactosidase from B. fragilis is a novel galactosidase belong to CAZy GH110 which can clear the terminal αGal from branched and linear oligosaccharides. This study was purposed to investigate the removal effect of a novel α-galactosidase on α-Gal XTA on surface of red blood cells. The αGal XTA from the red blood cells of cattle, pig, dog and rabbit was digested by using recombinant α-galactosidase; the α-Gal antigens on surface of cells was detected by flow cytometry. The results showed that the XTA was disappeared completely or mainly. It is concluded that the novel α-galactosidase is a potential enzyme to remove the XTA on the surface of xenotransplants and can be used to overcome the HAR in xenotransplantation.
