Discovery of a novel A2 allel in ABO blood group system and investigation of its distribution in Han population of Chinese Fujian province.
- Author:
Ai ZHANG
1
;
Quan CHI
;
Ben-Chun REN
Author Information
1. Fujian Provincial Blood Center, Fujian Province, China.
- Publication Type:Journal Article
- MeSH:
ABO Blood-Group System;
genetics;
Alleles;
Asian Continental Ancestry Group;
genetics;
Base Sequence;
Ethnic Groups;
genetics;
Genetics, Population;
Genotype;
Humans;
Sequence Analysis, DNA
- From:
Journal of Experimental Hematology
2012;20(5):1243-1245
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the distribution of A2 subgroup in Han Population of Chinese Fujian province and its molecular mechanisms. One individual with serologic ABO blood grouping discrepancy was identified with commercially available monoclonal and polyclonal antibodies and lectin: anti-A, anti-B, anti-AB, anti-A1, and anti-H reagents according to the routine laboratory methods. DNA sequences of exon 6, 7 and intron 6 of ABO gene were analyzed by polymerase chain reaction using genomic DNA and direct DNA sequencing or sequencing after gene cloning. Red cells of 3 176 A or AB unrelated individuals were tested with anti-A1. The results showed that this individual was identified as A2 subgroup by serological technology, sequencing analysis indicated the A2 subgroup with novel A variant allele, the novel A allele being different from the allele A101 by 467C > T and 607G > A missense mutation in exon 7, no A2 subgroup was identified from the 3 176 individuals by using standard serological technology. It is concluded that a novel A allele responsible for A2 subgroup composing of 467C > T and 607G > A has been firstly confirmed, and the A2 subgroup is very rare in Chinese Fujian Han population.
