Patterns of Intrahepatic Gene Expression in Neonatal Cholestasis.
- Author:
BoHwa CHOI
1
;
Byung Ho CHOE
;
Eun Jung CHUNG
;
Kyung Mo KIM
;
Heng Mi KIM
;
Jin Young PARK
;
Woo Hyun PARK
;
Moon Kyu KIM
;
Jung Chul KIM
Author Information
1. Department of Pediatrics, Seongsim General Hospital, Yeosu, Korea.
- Publication Type:Original Article
- Keywords:
Neonatal cholestasis;
Biliary atresia;
Gene expression;
cDNA microarray analysis
- MeSH:
Biliary Atresia;
Biopsy, Needle;
Cholestasis*;
Clone Cells;
Diagnosis;
DNA, Complementary;
Fluorescence;
Gene Expression*;
Hepatitis;
Humans;
Liver;
Liver Transplantation;
Oligonucleotide Array Sequence Analysis;
RNA;
Tissue Donors;
Transcriptome
- From:Korean Journal of Pediatric Gastroenterology and Nutrition
2005;8(2):177-193
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To identify genes specifically expressed in biliary atresia, we compared the patterns of gene expression between biliary atresia and neonatal hepatitis syndrome using cDNA microarray analysis. METHODS: Liver tissues were taken from livers of 11 patients (7 patients with biliary atresia and four with neonatal hepatitis) with neonatal cholestasis by needle biopsy. Normal control could be obtained from donor liver tissue during living-related liver transplantation. Total RNA was extracted from each samples and reversely transcribed to make cDNA. Then fluorescent cDNA were pooled and hybridized to the clones on the microarray. Fluorescence intensities at the immobilized targets were measured. Utilizing cDNA arrays of 4.7 K human genes, gene expression profiles were analyzed. RESULTS: Among 4,700 microarray clones, 17 cDNA clones were significantly over-expressed in all 11 patients with neonatal cholestasis, while 20 clones were significantly decreased. Genome-wide expression analysis was carried out in livers obtained at the time of diagnosis. We could identify 49 genes, in which there showed differential expression between biliary atresia and neonatal hepatitis syndrome. CONCLUSION: This study shows the pattern of differentially expressed genes in biliary atresia and neonatal hepatitis syndrome. We believe that this study can contribute to the understanding of pathogenesis of neonatal cholestasis.
