Cytomegalovirus-specific T cells immune reconstitution after human leukocyte antigen matched sibling donor allogeneic bone marrow plus peripheral blood hematopoietic stem cell transplantation.
- Author:
Xiao-hua LUO
1
;
Ying-jun CHANG
;
Ming-rui HUO
;
Dan LI
;
Xiao-jun HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Cytomegalovirus; immunology; Female; HLA Antigens; immunology; Hematologic Diseases; immunology; surgery; Hematopoietic Stem Cell Transplantation; Humans; Male; Middle Aged; Peripheral Blood Stem Cell Transplantation; Siblings; T-Lymphocytes, Cytotoxic; immunology; Tissue Donors; Young Adult
- From: Chinese Journal of Hematology 2012;33(8):605-609
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the regular pattern of Cytomegalovirus (CMV)-specific T cells (CTL) immune reconstitution after human leukocyte antigen (HLA) matched sibling donor allogeneic bone marrow(BM) plus peripheral blood hematopoietic stem cell (PBSC) transplantation.
METHODSCTL from seventeen patients after transplantation was detected by flow cytometry, the IFN-γ secretion ability of CTL by enzyme-linked immunospot (ELISPOT) assay, and clonal analysis of TCR Vβ subfamily by gene scan assays. The relationship between CTL reconstitution and CMV infection was studied.
RESULTSBoth number and function of recipients CTL reached to normal control level at 30 d post-transplantation. The recipients achieved a high frequency CTL with IFN-γ response and restoration of T-cell receptor β (TCR Vβ) repertoire at one year post-transplantation. CTL with the central memory CD45RO(+)CD62L(+) cell phenotype expanded in PB when CMV was reactivated. The incidence of CMV reactivation was 35.83% (17.91% - 63.10%) after transplantation, and none of them developed CMV disease.
CONCLUSIONAfter HLA matched related donor transplantation using mixed grafts, immune recovery to CMV seems to be early and fast. The incidence of CMV infection and disease are low.
