Biological characteristics of bone marrow mesenchymal stem cells and JAK2 mutation in myeloproliferative neoplasms.
- Author:
Hong TIAN
1
;
Guang-Hua CHEN
;
Yang XU
;
Rong-Xian WANG
;
Man QIAO
;
Hui-Wen LIU
;
De-Pei WU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Aged, 80 and over; Bone Marrow Cells; cytology; Bone Marrow Neoplasms; genetics; Case-Control Studies; Child; DNA Mutational Analysis; Female; Humans; Janus Kinase 2; genetics; Male; Mesenchymal Stromal Cells; cytology; Middle Aged; Mutation; Myeloproliferative Disorders; genetics; Young Adult
- From: Chinese Journal of Hematology 2012;33(9):701-704
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the biological characteristics of bone marrow mesenchymal stem cells (BMSCs) and detect JAK2 mutation in BMSCs from myeloproliferative neoplasms (MPN) patients.
METHODSJAK2 V617F mutation and exon 12 mutation in 70 MPN patients' blood or bone marrow samples were detected. Isolated BMSCs were then characterized their phenotype, mesenchymal differentiation capacity and existence of JAK2 mutation.
RESULTSBMSCs derived from the patients were similar with healthy donors in terms of morphology, surface antigen and differentiation ability. Of them, 38 patients' blood or bone marrow samples harbored JAK2 V617F, and identified that 3 V617F-negative-patients' samples existed JAK2 exon 12. No patients' BMSC harbored JAK2 mutation though their blood or bone marrow samples carried JAK2 mutation.
CONCLUSIONBMSCs from MPN patients had similar biological characteristics with healthy donors. BMSCs from MPN patients known to bear JAK2 mutation in blood or bone marrow cells didn't carry the mutation.
