Identification and analysis of absorbed components in rat plasma after oral administration of active fraction of Corydalis yanhusuo by LC-MS/MS.
- Author:
Xing-Ye CHENG
1
;
Yue SHI
;
Hong SUN
;
Wen JIN
;
Shun-Liang ZHENG
;
Kai-Tong LI
;
Shuai YANG
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
- Publication Type:Journal Article
- MeSH:
Administration, Oral;
Alkaloids;
administration & dosage;
blood;
isolation & purification;
metabolism;
Animals;
Chromatography, High Pressure Liquid;
Corydalis;
chemistry;
Drugs, Chinese Herbal;
administration & dosage;
isolation & purification;
metabolism;
Glucuronides;
blood;
Male;
Plants, Medicinal;
chemistry;
Rats;
Rats, Wistar;
Spectrometry, Mass, Electrospray Ionization;
Tandem Mass Spectrometry
- From:
Acta Pharmaceutica Sinica
2009;44(2):167-174
- CountryChina
- Language:Chinese
-
Abstract:
To analyze and identify the constituents in rat plasma after oral administration of the active fraction of Corydalis yanhusuo, a LC-MS/MS method was established. The constituents absorbed into blood, their original crude drugs and their metabolites were identified either by comparing the retention time and mass spectrometry data with that of reference compounds or by mass spectrometry analysis and retrieving the reference literatures. Nine species are the original form in Corydalis yanhusuo, moreover, some metabolites in blood identified as glucuronide were found. The constituents absorbed into blood and the possible metabolites which demonstrate to originate from the active fraction of Corydalis yanhusuo are responsible for the observed efficacy. Its serum pharmacochemistry should be subjected to complete investigation so as to illuminate the pharmacology and active mechanism of the active fraction of Corydalis yanhusuo.
