Annotation of complete genomic sequence of 3p24-p25 478 kb of human DNA.
- Author:
Ke-yue DING
1
;
Yi-lin ZHANG
;
Li-hong CHEN
;
Yan SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Chromosome Mapping; Chromosomes, Artificial, Bacterial; Chromosomes, Human, Pair 3; Genome, Human; Human Genome Project; Humans; Molecular Sequence Data
- From: Acta Academiae Medicinae Sinicae 2002;24(3):259-263
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo annotate the human genome 3p24-p25 478 kb complete sequence.
METHODSThe protein-coding genes in the genomic sequence were identified by using ab initio gene finding, homology-based similarity database searching and all or partial mRNA aligning with genomic sequence, and the content feature of the genomic sequence were analyzed by using EMBOSS package.
RESULTSTwo known genes SLC6A1 and SLC6A11 were identified; as well as the GC content of this genomic sequence was 47% and 3 putative CpG islands were predicted in the genomic sequence, located in 130,685-131,516 bp, 307,090-307,870 bp and 415,585-416,308 bp, respectively.
CONCLUSIONSThe methods, as mentioned above, might be used for annotating the biological information in the genomic sequence, such as gene structure, GC content, CpG island.
