Cloning cDNA encoding for GAGA-like element binding proteins in human Jurkat cells.
- Author:
Zhi-cheng MO
1
;
Yu LU
;
Chao GAO
;
Ning-hua WU
;
Yu-fei SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Carrier Proteins; biosynthesis; genetics; Cloning, Molecular; DNA, Complementary; genetics; DNA-Binding Proteins; Drosophila Proteins; HSP90 Heat-Shock Proteins; biosynthesis; genetics; Homeodomain Proteins; genetics; Human T-lymphotropic virus 1; genetics; Humans; Jurkat Cells; Leukemia, T-Cell; metabolism; pathology; Transcription Factors; genetics; Two-Hybrid System Techniques
- From: Acta Academiae Medicinae Sinicae 2002;24(3):269-271
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore GAGA-like element binding protein in human cells.
METHODSYeast one-hybrid system was used to screen the GAGA-like element binding proteins in HTLV-1 transformed Jurkat cell cDNA fusion library. Total RNA extracted from Jurkat cells was first labeled by reverse transcription, and was taken as cDNA probe to hybridize with the candidate positive clones.
RESULTS9 positive clones were obtained, and 6 out of the 9 clones were positively hybridized with the cDNA probe.
CONCLUSIONS6 candidate clones encoding for GAGA-like element binding proteins were obtained from Jurkat cells for further investigation.
