Establishment and application of a RT-PCR system for quantifying mRNA of 4 hsp genes in human cells.
- Author:
Hong-fan LI
1
;
Xing-rong LIU
;
Feng-yan HENG
;
Ning-hua WU
;
Yu-fei SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Chaperonin 60; biosynthesis; genetics; HSP40 Heat-Shock Proteins; HSP70 Heat-Shock Proteins; biosynthesis; genetics; HSP90 Heat-Shock Proteins; biosynthesis; genetics; Heat-Shock Proteins; biosynthesis; genetics; Humans; Leukemia, T-Cell; metabolism; pathology; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction; Tumor Cells, Cultured
- From: Acta Academiae Medicinae Sinicae 2002;24(3):321-324
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a RT-PCR system for detecting mRNA expression level of 4 hsp genes in human cells.
METHODSRT-PCR system was established with gene cloning, gene recombination, in vitro transcription and RT-PCR techniques; The detection of expression level of 4 hsp genes in SW13 cell was carried out with this system.
RESULTSIn SW13 cell, hsp70 and hsp90 alpha were typical heat shock induced genes, while hsp60 and hsp90 beta were efficiently expressed and further induced by heat-shock to various extent.
CONCLUSIONSIn our hands novel RT-PCR system can be used to detect mRNA expression level of 4 human hsp genes.
