An observation of taurine transport alterations in calcification of myocardial cells in vitro.
- Author:
Yan-rong SHI
1
;
Shu-heng WANG
;
Ding-fang BU
;
Yong-fen QI
;
Lin GAO
;
Yong-zheng PANG
;
Chao-shu TANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biological Transport; Calcinosis; metabolism; pathology; Calcium; metabolism; Carboxy-Lyases; metabolism; Cells, Cultured; Myocytes, Cardiac; metabolism; pathology; RNA, Messenger; metabolism; Rats; Taurine; biosynthesis; genetics; metabolism
- From: Acta Academiae Medicinae Sinicae 2002;24(4):359-363
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the alterations of taurine transport, taurine transporter (TAUT) and cysteine sulfinate decarboxylase (CSD) mRNA in the calcification of myocardial cells in vitro.
METHODS3H-taurine measured the amount of taurine uptake. TAUT and CSD mRNA consents were measured using competitive quantitative RT-PCR in cultured and calcified myocardial cells.
RESULTSIn calcification of myocardial cells, taurine concentration was decreased by 27% (P < 0.05), taurine uptake was markedly reduced, Vmax reduced by 39% (P < 0.01), there were no statistical significance of Km values between the two groups. TAUT mRNA decreased by 45% (P < 0.01), but CSD mRNA increased by 25% (P < 0.05).
CONCLUSIONSThe data suggest that there were impediment of taurine transport in calcification of myocardial cells, as TAUT mRNA level was decreased, but CSD mRNA concentration was improved.
