Construction of a collagen gel dermis with improved biological property.
- Author:
Ling-rong LIU
1
;
Li-hai ZHANG
;
Dong-rui MA
;
Qi-qing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Separation; Cells, Cultured; Collagen Type I; biosynthesis; Dermis; cytology; metabolism; Extracellular Matrix Proteins; biosynthesis; Fibroblasts; cytology; Humans; Infant, Newborn; Male; Skin, Artificial; Tissue Engineering
- From: Acta Academiae Medicinae Sinicae 2002;24(4):373-376
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo improve the biological property of artificial skin.
METHODSWe have ameliorated Hansburgh and Middelkoop's method of manufacturing artificial dermis. The type I collagenase and Dispase were used to isolated neonate prepuce' dermis fibroblast. The gel dermis was constructed by compounding the fibroblast and collagen swelling solution. The property of the collagen gel dermis was measured.
RESULTSThe neonate prepuce's dermis fibroblast had property of high proliferation, high activation of the dermis, and it could secrete abundant extracellular matrix (ECM).
CONCLUSIONThe collagen gel dermis is an useful dermis substitute.
