Effect of alpha-difluoromethylornithine on growth characteristics and expression of ALT-04ag gene of human lung carcinoma cells.
- Author:
Zhi-hua CHEN
1
;
Hai-ping KE
;
Hong-yan LI
;
Qing FANG
;
Shu-lan CAO
;
Mu-zhen FAN
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Neoplasm; biosynthesis; genetics; Apoptosis; Carcinoma, Squamous Cell; pathology; Cell Division; Down-Regulation; Eflornithine; pharmacology; Gene Expression Regulation, Neoplastic; Humans; Lung Neoplasms; pathology; Oncogene Proteins; genetics; RNA, Messenger; biosynthesis; genetics; Tumor Cells, Cultured
- From: Acta Academiae Medicinae Sinicae 2002;24(4):377-380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of polyamine biosynthesis inhibition on growth characteristics of human lung carcinoma cells and its correlation with the expression of human lung carcinoma associated antigen ALT-04ag gene.
METHODSThe gene expression was detected by RT-PCR and immunocytochemical tests. The cell growth characteristics were studied by cell growth curves, morphological observation, FCM analysis and DNA electrophoresis.
RESULTSHuman lung squamous carcinoma cells L78 treated with 5 mmol/L alpha-difluoromethylornithine (DFMO) for 5 days showed significant growth inhibition and apoptosis induction. The mRNA and protein expressions of ALT-04ag gene in the cells were downregulated, while these changes resulted from DFMO treatment were prevented by provision of DFMO along with exogenous putrescine.
CONCLUSIONThe effect of polyamine biosynthesis inhibition induced by DFMO restrains the growth characteristics and promotes apoptosis of human lung carcinoma L78 cells, which is associated with down regulation of ALT-04ag gene expression.
