Expression and purification of gene transfer vehicle mediated by epidermal growth factor receptor.
- Author:
Hua-sheng FANG
1
;
Lin ZHANG
;
Sheng-dong LU
;
Shu-zheng ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Base Sequence; Escherichia coli; genetics; Gene Transfer Techniques; Genetic Therapy; Genetic Vectors; Histones; biosynthesis; genetics; Humans; Molecular Sequence Data; Protein Engineering; Receptor, Epidermal Growth Factor; biosynthesis; genetics; Recombinant Fusion Proteins; biosynthesis; genetics; isolation & purification
- From: Acta Academiae Medicinae Sinicae 2002;24(4):381-384
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo create a gene transfer vehicle for targeting gene therapy of cancer with epidermal growth factor receptor overexpressing.
METHODSEncoding sequences of the first domain of histone gene (H1) and EGF C-loop (EGFc) were obtained by PCR amplification. These two DNA fragments were ligated by EcoR I site, and cloned and sequenced. E. coli expression vector of the fusion gene was then constructed. The fusion protein H1EGFc was purified by specific band isolation from SDS-PAGE.
RESULTSThe molecular weight of purified protein was consistent with the designed request. Its purity reached 94.02%.
CONCLUSIONA fusion protein H1EGFc was expressed and purified.
