Architecture, influencing factors, and sensitivity to antifungal agents of Candida biofilm.
- Author:
Qiu-ning SUN
1
;
Kai FANG
Author Information
- Publication Type:Journal Article
- MeSH: Amphotericin B; pharmacology; Antifungal Agents; pharmacology; Biofilms; drug effects; Candida; drug effects; ultrastructure; Drug Resistance, Fungal; Fluconazole; pharmacology; Itraconazole; pharmacology; Microbial Sensitivity Tests; Microscopy, Confocal
- From: Acta Academiae Medicinae Sinicae 2002;24(4):385-388
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a model of Candida biofilm and to explore its characteristics, ultrastructure, influences by saliva and serum, and sensitivity to antifungal agents.
METHODSEvaluations of the in vitro growth kinetics, influences by saliva and serum, and sensitivity to antifungal agents of Candida biofilm were performed with the abated tetrazolium salt XTT method on a 96-well microtire petri dish. The ultrastructure of Candida biofilm was observed under Confocal Laser Scanning Microscope (CLSM).
RESULTSThe bioactivity of Candida biofilm increased with culturing time and serum could obviously increase the action of biofilm. The Candida biofilm was significantly resistant to routine antifungal agents.
CONCLUSIONThe Candida cells adhered in biofilms are significantly different in morphology from those in suspension and are resistant to routine antifungal agents such as Amphotericine B, Fluconazole and Itraconazole.
