Genotyping of HLA-DRB1 by PCR-SSP in Yunnan Lahu ethnic groups.
- Author:
Kun-xue HONG
1
;
Qin HE
;
Xi-yun SAN
;
Jian-kun YU
;
Ke-qin LIN
;
Lei SHI
;
Jia-you CHU
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; DNA Primers; Ethnic Groups; Female; Gene Frequency; Genotype; HLA-DR Antigens; genetics; HLA-DRB1 Chains; Humans; Male; Polymerase Chain Reaction; methods
- From: Acta Academiae Medicinae Sinicae 2002;24(4):389-392
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate polymorphism of HLA-DRB1 in Chinese Lahu population in Yunnan.
METHODSPolymerase chain reaction-sequence specific primers (PCR-SSP) were used to determine HLA-DRB1 genotypes of 110 unrelated healthy Lahu minority people of Yunnan Province.
RESULTSSixteen alleles of DRB1 were detected in this study. The results of test showed that the genotype distributions observed were corresponded with the Hardy-Weinberg equilibrium.
CONCLUSIONThis study has obtained a more comprehensive and accurate data set of the normal allele frequencies of HLA-DRB1 in Chinese Lahu population in Yunnan Province, which may be of significance in the studies on population genetics and disease association.
