Study on the function of HSD-3.8 gene encoding a testis-specific protein with yeast two-hybrid system.
- Author:
Wen LIN
1
;
Shi-ying MIAO
;
Lin ZHANG
;
Lin-fang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenosine Triphosphate; metabolism; Adult; Antigens, Surface; DNA-Binding Proteins; genetics; Female; GTP-Binding Proteins; genetics; physiology; Gene Library; Humans; Male; Protein Biosynthesis; Proteins; genetics; Spermatozoa; chemistry; physiology; Synaptophysin; Testis; chemistry; Two-Hybrid System Techniques; Yeasts; genetics
- From: Acta Academiae Medicinae Sinicae 2002;24(6):582-587
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the protein factors that could interact with the testis-specific protein encoded by HSD-3.8 gene (GenBank Accession Number AF311312) related with female fertilization.
METHODSYeast two-hybrid system was used to screen the human ovary MATCHMAKER cDNA library with constructed "bait plasmid" containing the 0.7 kb fragment (HSD-0.7) of HSD-3.8. The interaction with the positive fragments using a series of truncated bait plasmids was investigated.
RESULTSOne positive gene fragment was obtained, which coded for 144 amino acids of the C-terminus of human G protein beta subunit 1. Truncated bait plasmids couldn't interact with the fish protein fragment in yeast.
CONCLUSIONSThe protein encoded by HSD-3.8 gene may function through G protein signal transduction pathway and the interaction depends on the integration of the bait protein.
