Establishment of drug-resistance cell line of human glioma mediated by MGMT.
- Author:
Peng JIN
1
;
Qing-lin ZHANG
;
Fu-sheng LIU
;
Bao-an WANG
;
Lin WEI
;
Cheng-wei WANG
;
Jian-xin KONG
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; biosynthesis; Brain Neoplasms; pathology; Cell Line, Tumor; DNA Modification Methylases; biosynthesis; genetics; Drug Resistance, Neoplasm; genetics; Glioma; pathology; Humans; O(6)-Methylguanine-DNA Methyltransferase; metabolism; physiology
- From: Acta Academiae Medicinae Sinicae 2002;24(6):596-600
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a drug-resistance cell line of human glioma mediated by MGMT.
METHODSSimulated the clinical usage of BCNU to establish a BCNU-resistant human glioma subline by cyclic exposing the U251 parent cells to a constant concentration of BCNU. The resistance index and the expression of MGMT mRNA of U251/BCNU were detected and compared the difference of in vitro proliferation between U251 and U251/BCNU.
RESULTSA subline--U251/BCNU was successfully established in about 4-month culture, which had a stable resistance to BCNU. U251/BCNU cells showed 17-fold higher resistance to BCNU than did U251 cells by MTT assay, while U251/BCNU cells expressed MGMT mRNA. The doubling time of U251 and U251/BCNU had no statistical difference.
CONCLUSIONA drug-resistance cell line of human glioma mediated by MGMT is established, which could provide experimental basis for further studies on the resistance mechanism and reversal methods of glioma chemotherapy.
