Genetic polymorphism of 9 STR loci in Han nation population in Shandong Province.
- Author:
Bo-feng ZHU
1
;
Gui-ping LÜ
;
Chun-mei SHEN
;
Gui-fa YAO
;
Ying-fang TIAN
;
Tao LI
;
Zhen-yuan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; China; ethnology; Gene Frequency; Genetic Markers; genetics; Genotype; Humans; Polymorphism, Genetic; Repetitive Sequences, Nucleic Acid; Tandem Repeat Sequences
- From: Acta Academiae Medicinae Sinicae 2002;24(6):620-625
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study genetic polymorphism of 9 STR loci in Han nation population in Shangdong Province.
METHODSWe investigated 100 unrelated individuals of Han nation population in Shandong Province and studied their genetic distribution of 9 STR loci and Amelogenin locus. Primers of 10 loci were labeled with the NHS-ester fluorescent dye 5-FAM (blue), Joe (green) or NED (yellow). The data of Han nation population were generated by multiple amplification and subjected to GeneScan, genotype and genetic distribution analysis.
RESULTS83 alleles and 220 genotypes were observed, with the corresponding frequency of 0.0050-0.4050 and 0.0100-0.2100. The average of heterozygosity was 0.7778, the accumulated discrimination power was 0.9999. The accumulated probability of exclusion paternity was 0.9999. The polymorphism information content was 0.5823-0.8396.
CONCLUSIONSChi-Square test indicated that the distribution of genotypes agreed with Hardy-Weinberg equilibrium.
