Molecular cloning of Plasmodium yoelii dynamin-like protein (PyDyn) gene and the immunological character of its domains.
- Author:
Dong WANG
1
;
Ying-hong MAO
;
Heng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; Dynamins; genetics; immunology; Escherichia coli; genetics; Genes, Protozoan; genetics; immunology; Malaria Vaccines; immunology; Molecular Sequence Data; Plasmodium yoelii; chemistry; genetics; Protozoan Proteins; genetics; immunology; Vaccines, Synthetic; immunology
- From: Acta Academiae Medicinae Sinicae 2003;25(2):176-180
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify and clone a new full ORF gene of PyDyn (Plasmodium yoelii dynamin-like protein), and examine the protection of their expression products.
METHODUsing the P. yoelii Genome technology and RT-PCR.
RESULTSThe full ORF gene of PyDyn was amplified from mRNA of the erythrocytic stage of P. yoelii., three domains of PyDyn were expressed in E. coli., and the fairly positive immunogenicity of them was showed by IFA. The full ORF gene of PyDyn was 2,433 bp and encode 811 amino acids. Its Gene Bank access number is AF458071. PyDyn belongs to the dynamin-like protein family according to its property.
CONCLUSIONThe new full ORF gene of PyDyn is obtained and identified; their expressed domains are probably new candidates for malaria vaccine.
