Gene clone and its characteristics on band 7-like protein in Plasmodium falciparum FCC1/HN.
- Author:
Ling ZHANG
1
;
Lian-hui ZHANG
;
Hai-yi WANG
;
Heng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Blood Proteins; biosynthesis; genetics; immunology; DNA, Complementary; genetics; Membrane Proteins; biosynthesis; genetics; immunology; Molecular Sequence Data; Phylogeny; Plasmodium falciparum; chemistry; genetics; pathogenicity; Protozoan Proteins; biosynthesis; genetics; immunology; Rabbits; Recombinant Proteins; biosynthesis; genetics
- From: Acta Academiae Medicinae Sinicae 2003;25(2):181-184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify and clone the gene named pfstom gene which encoding the protein belonging to band 7 family and to do primary research on its function.
METHODSBased on the finished data in international public malaria database, coding sequence of pfstom cDNA was obtained by RT-PCR from FCC1/HN. Its phylogenetic profiles and the homogeny were analyzed by some softwares. After Prokaryotic expression, C terminal of Pfstom protein was expressed by Pet30a system. Recombinant Pfstom protein was used to immol/Lunize rabbit and then serum was harvested and the IgG was purified for Western blot.
RESULTSThe coding sequence of pfstom is 1,125 bp which encoding 374 amino acids with C-terminal fragment being homogenous to stomatin-like protein which belongs to band 7 family. Phylogenetic profiles analysis revealed its homogeny to stomatin. Western blot showed its stage-specific expression in trophozoite.
CONCLUSIONPfstom belongs to band-7 family. It was expressed specifically in trophozoite in erythrocyte stage of plasmodium falciparum. It was not expressed in ring stage. And it is membrane-related protein. All these results provided the foundation for further research on pfstom.
