Effect of astragalus-angelica mixture on osteopontin expression in rats with chronic nephrosclerosis.
- Author:
Ya-ni ZHAO
1
;
Jing-zi LI
;
Ling YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Astragalus Plant; Drug Synergism; Drugs, Chinese Herbal; pharmacology; Male; Nephrosclerosis; chemically induced; metabolism; Osteopontin; Phytotherapy; Puromycin; Rats; Rats, Sprague-Dawley; Sialoglycoproteins; biosynthesis
- From: Chinese Journal of Integrated Traditional and Western Medicine 2002;22(8):613-617
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Astragalus-Angelica Mixture (AAM) on osteopontin (OPN) expression in rats with chronic nephrosclerosis.
METHODSChronic nephrosclerosis model rats induced by repeated intraperitoneal injection of puromycin were randomly divided into the model group, AAM group and Irbesartan (an antagonist of angiotensin) group. The experimental course lasted 12 weeks. Blood and urine samples were examined by biochemical method. Kidney tissue was taken for pathological stain and immunohistochemical method and was applied to examine OPN expression, mononuclear macrophage, laminin in extracellular matrix and decorin expressions.
RESULTSAAM showed the effects of decreasing urinary protein and improving renal function similar to that of Irbesartan. It also could alleviate the pathological damage of kidney tissue, especially in decreasing renal tubular mesenchymal damage index. The accumulation of decorin and laminin in the mesenchymal extracellular matrix significantly decreased. Renal tubular OPN expression and mesenchymal infiltration of mononuclear macrophage decreased significantly and in a positive correlated manner (r = 0.885, P < 0.01).
CONCLUSIONAAM has similar renal protective action to that of Irbesartan, this action may be related to the inhibition of up-regulated OPN expression.
