Effects of antihistamine treatment on immune function in rats with experimental hepatitis.
- Author:
Hong LI
1
;
Long-feng ZHAO
;
Yan-qin HAO
;
De-wu HAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Ascorbic Acid; pharmacology; Disease Models, Animal; Hepatitis; immunology; metabolism; therapy; Histamine Antagonists; pharmacology; Interferon-gamma; metabolism; Interleukin-10; metabolism; Interleukin-12; metabolism; Interleukin-4; metabolism; Ketotifen; pharmacology; Liver; drug effects; metabolism; Male; Rats; Rats, Wistar; Th1-Th2 Balance; Tumor Necrosis Factor-alpha; metabolism
- From: Chinese Journal of Hepatology 2013;21(10):764-768
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of antihistamine treatment on immune function in rats with experimental hepatitis.
METHODSThirty Wistar rats were randomly allocated into three groups:experimental hepatitis group (EH group), antihistamine treatment group (AH group) and normal control group (NC group). Rats in the EH group received the subcutaneous injection of 40% carbon tetrachloride oil solution and were fed on diet with low-protein, low-choline, high-fat and high-alcohol,while rats in the AH group received antihistamine treatment(ketotifen + vitamin C) additionally.They were sacrificed after 4 weeks, and the levels of serum alanine aminotransferase(ALT), total bilirubin (TBil), histamine(HA), IFNgamma, IL-12, IL-4 and IL-10 were determined. The levels of IL-12 mRNA and IFN-gamma mRNA in liver tissue were determined via real-time reverse transcriptional polymerase chain reaction(RT-PCR).
RESULTS(1) Compared to the NC group, in the EH group, the levels of ALT, TBil, and circulating and intrahepatic HA were significantly increased(P less than 0.05); intrahepatic HA were significantly decreased(P less than 0.05) after antihistamine treatment. (2) Compared to the NC group, in the EH group, the levels of IL-4, IL-10 were significantly increased((0.504+/-0.202)ng/ml and (29.025+/-1.478) pg/ml vs (0.811+/-0.244)ng/ml and (33.72+/-4.293)pg/ml respectively, P less than 0.05), and the levels of IL-12 were decreased ((6.515+/-2.893)pg/ml vs (3.519+/-1.113)pg/ml, P less than 0.05); and after antihistamine treatment the levels of IL-4 and IL-10 were significantly decreased (were (0.423+/-0.168)ng/ml and (30.412+/-3.275)pg/ml, P less than 0.05), the levels of IL-12 were significantly increased (P less than 0.05), but the level of IFNgamma had no significance (P more than 0.05). The levels of intrahepatic IL-12 mRNA and IFNgamma mRNA had similar results.
CONCLUSIONAntihistamine treatment may improve liver function and correct Th1/Th2 unbalance.
