Changes of Regulatory T Cells in the Early Stage of Obesity Mice and Their Modulation on Macrophage Subtypes in Visceral Adipose Tissue.
10.3881/j.issn.1000-503X.2016.04.006
- Author:
Xia LI
1
;
Xiao-Han TANG
1
;
Li-Li TANG
1
;
Hai-Bo YU
1
;
Zhi-Guo XIE
1
;
Zhi-Guang ZHOU
1
Author Information
1. Department of Metabolism and Endocrinology,the Second Xiangya Hospital,Central South University,Key Laboratory of Diabetes Immunology,Ministry of Education,Central South University,National Clinical Research Center for Metabolic Diseases,Changsha 410011,China.
- Publication Type:Journal Article
- MeSH:
Animals;
Blood Glucose;
Diet, High-Fat;
Inflammation;
Intra-Abdominal Fat;
cytology;
Leptin;
blood;
Macrophages;
cytology;
Mice;
Mice, Inbred C57BL;
Mice, Obese;
Obesity;
immunology;
T-Lymphocytes, Regulatory;
cytology
- From:
Acta Academiae Medicinae Sinicae
2016;38(4):399-403
- CountryChina
- Language:English
-
Abstract:
Objective To investigate the changes of regulatory T cells (Tregs) and whether Tregs can modulate the distribution of macrophage subtypes in visceral adipose tissue in the early stage of obesity.Methods After C57BL/6 mice obesity models were successfully established,metabolic parameters and numbers of Tregs and M1/M2 macrophage were measured at 4,10,and 20 weeks.The changes of metabolic parameters and adipose tissue inflammation in obesity mice after rapamycin intervention were evaluated. Results The early-stage obesity models were successfully established.Compared with normal diet mice,high fat diet mice had significantly higher epididymal adipose tissue mass and serum leptin levels(P<0.05).However,there was no statistical difference in blood glucose and insulin levels between these two groups(All P>0.05). Macrophages infiltration in adipose tissue in high fat diet mice gradually increased with time,coincident with decrease in Treg numbers. Increased numbers of Treg,improved metabolic parameters,and decreased ratio of M1/M2 can be seen after rapamycin intervention in mice.Conclusion The decrease of Tregs in the early stage of obesity may contribute to abnormal distribution of macrophage subtypes in visceral adipose.
