Establishment of An Alloxan-induced Diabetes Model in Daphnia Pulex.
10.3881/j.issn.1000-503X.2016.06.007
- Author:
An-Min HU
1
;
Tao ZHU
1
;
Ye JIANG
2
;
Li DONG
2
;
Hong GAO
2
;
Gui-Zhi DU
1
Author Information
1. Laboratory of Anesthesia and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
2. Department of Anesthesiology, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China.
- Publication Type:Journal Article
- MeSH:
Alloxan;
Animals;
Blood Glucose;
analysis;
Daphnia;
Diabetes Mellitus, Experimental;
chemically induced;
physiopathology;
Disease Models, Animal;
Gene Expression Regulation;
Glucose Transport Proteins, Facilitative;
genetics;
metabolism;
Likelihood Functions;
Phylogeny;
Real-Time Polymerase Chain Reaction
- From:
Acta Academiae Medicinae Sinicae
2016;38(6):660-665
- CountryChina
- Language:English
-
Abstract:
Objective To establish a Daphnia model of alloxan-induced diabetes. Methods Daphnia were exposed to three different concentrations of alloxan (3, 5, and 10 mmol/L) for 30 minutes. Blood glucose and survival rate were recorded for 72 hours after alloxan insult. Sequence analysis and phylogenetic inference for glucose transporters (GLUT) were clustered with the maximum-likelihood method. Using reverse transcription and quantitative polymerase chain reaction techniques, we investigated the transcriptional changes of GLUT at 12 hours after alloxan (5 mmol/L) exposure. Results Compared with control, 3 mmol/L, and 5 mmol/L as well as 10 mmol/L alloxan initially induced transient blood glucose decline by 15% for 2 hours and 12 hours respectively. In Daphnia with 5 and 10 mmol/L alloxan, their blood glucose was persistently raised by about 150% since after 24-hour insult. Survival rate of Daphnia exposure to alloxan with concentrations of 3, 5, and 10 mmol/L were 90%, 75%, and 25% respectively. We predicted seven GLUT genes in the Daphnia genome and successfully amplified them using real-time polymerase chain reaction. Two of seven GLUT transcripts were down-regulated in Daphnia with 5 mmol/L alloxan-induced diabetes. Conclusion Alloxan-induced diabetes model was successfully established in the Daphnia pulex, suggesting diabetes-relevant experiments can be conducted using Daphnia.
