Fluorescein Diacetate Microplate Assay in Cell Viability Detection.
10.3881/j.issn.1000-503X.2016.06.014
- Author:
Xi CHEN
1
;
Xiu-Ying YANG
1
;
Lian-Hua FANG
1
;
Guan-Hua DU
1
Author Information
1. Beijing Key Laboratory of Drug Target Identification and Drug Screening,Institute of Materia Medica, CAMS and PUMC,Beijing 100050,China.
- Publication Type:Journal Article
- MeSH:
Biological Assay;
Cell Survival;
Fluoresceins;
chemistry;
Fluorescence;
Humans;
Hydrogen Peroxide;
Staining and Labeling
- From:
Acta Academiae Medicinae Sinicae
2016;38(6):710-714
- CountryChina
- Language:English
-
Abstract:
Objective To investigate the application of the fluorescein diacetate (FDA) microplate assay in cell viability detection. Methods Cells were seeded in a 96-well culture plate until detection. After incubated with FDA,the plate was detected by fluorescence microplate analyzer. The effects of FDA incubation duration,concentration,and other factors on the assay's accuracy and stability were assessed. We also compared the results of FDA with methyl thiazolyl(MTT) in terms of cell numbers and HOinjury. Results Within 0-30 minutes,the fluorescence-cell number coefficient of FDA assay increased with duration and reached 0.99 in 27-30 minutes. The optimum concentration of final FDA in this study was 10-30 μg/ml. On cell viability detection,the result of FDA method was equivalent to MTT method. As to HOinjury assay,the sensitivity of FDA method was superior to MTT on the higher concentration HOtreatment due to a relative shorter duration for detection. Conclusion As a stable and reliable method,FDA is feasible for cell variability detection under varied conditions.
