Effect of cetirizine hydrochloride on the expression of substance P receptor and cytokines production in human epidermal keratinocytes and dermal fibroblasts.
- Author:
Ji-Yong LIU
1
;
Yong-Zhe ZHAO
;
Cheng PENG
;
Feng-Qian LI
;
Quan-Gang ZHU
;
Jin-Hong HU
Author Information
1. Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
- Publication Type:Journal Article
- MeSH:
Anti-Allergic Agents;
pharmacology;
Cell Line;
Cetirizine;
pharmacology;
Fibroblasts;
cytology;
metabolism;
Histamine H1 Antagonists, Non-Sedating;
pharmacology;
Humans;
Interferon-gamma;
metabolism;
Interleukin-1beta;
metabolism;
Interleukin-8;
metabolism;
Keratinocytes;
cytology;
metabolism;
Receptors, Neurokinin-1;
metabolism;
Substance P;
pharmacology
- From:
Acta Pharmaceutica Sinica
2008;43(4):383-387
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of cetirizine hydrochloride on the expression of neurokinin 1 receptor (NK-1R) and cytokines production induced by substance P (SP) in HaCaT cells (a human epidermal keratinocyte cell line) and dermal fibroblasts. The effect of cetirizine on the expression of NK-1R protein was detected by flow cytometry and Western blotting analysis. The modulation of cetirizine on the production of interferon (IFN)-gamma, interleukin (IL)-1beta, IL-6 and IL-8 in HaCaT cells and fibroblasts was measured by ELISA. The results showed that cetirizine significantly inhibited the expression of NK-1R in HaCaT cells and fibroblasts. SP induced the production of IFN-gamma, IL-1beta and IL-8 in both cell types. Cetirizine 1-100 micromol x L(-1) inhibited SP-induced IL-1beta and IL-8 production in HaCaT cells and fibroblasts, while had no effect on the production of IFN-gamma in both cells. Both SP and cetirizine had no effect on the secretion of IL-6 in HaCaT cells and fibroblasts. These findings suggest that cetirizine may be involved in the treatment of SP-induced skin inflammation by inhibiting the expression of substance P receptor and regulation the production of IL-1beta and IL-8 in epidermal keratinocyte and dermal fibroblasts.
