Determination of betamethasone in human plasma by liquid chromatography with tandem mass.
- Author:
Ting-Ting QU
1
;
Rui ZHANG
;
Ben-Jie WANG
;
Xiao-Yan LIU
;
Gui-Yan YUAN
;
Rui-Chen GUO
Author Information
1. Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, Jinan 250012, China.
- Publication Type:Journal Article
- MeSH:
Anti-Inflammatory Agents;
blood;
pharmacokinetics;
Area Under Curve;
Betamethasone;
blood;
pharmacokinetics;
Chromatography, Liquid;
methods;
Humans;
Injections, Intramuscular;
Male;
Spectrometry, Mass, Electrospray Ionization;
methods;
Tandem Mass Spectrometry;
methods;
Young Adult
- From:
Acta Pharmaceutica Sinica
2008;43(4):402-407
- CountryChina
- Language:English
-
Abstract:
A sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of betamethasone in human plasma. The analyte was isocratically eluted on a Venusil XBP C8 column (200 mm x 3.9 mm ID, 5 microm) with methanol-water mol x L(-1) ammonium formate) (80:20) at a flow rate of 0.4 mL x min(-1), and detected (containing 5 mmol x L(-1) ammonium formate) (80:20) at a flow rate of 0.4 mL x min(-1), and detected with a triple quad LC-MS/MS using ESI with positive ionization. Ions monitored in the multiple reaction monitoring (MRM) mode were m/z 393.3-->355.2 for betamethasone and m/z 361.3-->343.2 for prednisolone (IS). Betamethasone was extracted from 0.5 mL human plasma with ethyl acetate. The average recovery is 88.24% and the low limit of quantitation (LLOQ) was 0.5 ng x mL(-1). The 3-day validation study demonstrated excellent precision and accuracy across the calibration range of 0.5-80.0 ng x mL(-1). The method was successfully applied to the pharmacokinetic study of compound betamethason injection in healthy Chinese volunteers.
