Analysis of primary metabolites of alpha-tocopherol in human urine by liquid chromatography-mass spectrometry.
- Author:
Ying-Yu PAN
1
;
Ying YU
;
Gu-Xian LAI
;
Li-Cai ZHU
Author Information
1. Department of Chemistry, South China Normal University, Guangzhou 510006, China.
- Publication Type:Journal Article
- MeSH:
Administration, Oral;
Antioxidants;
administration & dosage;
pharmacokinetics;
Chromatography, High Pressure Liquid;
methods;
Humans;
Spectrometry, Mass, Electrospray Ionization;
methods;
Vitamin E;
pharmacokinetics;
alpha-Tocopherol;
metabolism;
urine
- From:
Acta Pharmaceutica Sinica
2008;43(6):634-639
- CountryChina
- Language:Chinese
-
Abstract:
To investigate primary metabolites of alpha-tocopherol in human urine, the urine samples of five healthy volunteers after oral administration of 250 mg vitamin E once a day for seven days were collected within 0 -6 h in the seventh day. The samples were purified through C18 solid-phase extraction cartridge and analyzed by liquid chromatography-tandem mass spectrometry. alpha-Tocopheronic acid, 2,5,7, 8-tetramethyl-2-(2'-carboxyethyl) -6-suphate-chroman (alpha-CEHC-sulphate), gamma-tocopheronolactone, and 2, 5, 7, 8-tetramethyl-2-(4', 8', 12'-trimethyl-12'-carboxy dodecanyl) -6-suphate-chroman were found in urine of volunteers as four primary metabolites of alpha-tocopherol. The method has shown to be promising for alpha-tocopherol detection with many desirable properties including high sensitivity and selectivity, thus providing a reliable pathway for further study in metabolism of alpha-tocopherol.
