Preparation and application of antibody against staphylococcal enterotoxin C2.
- Author:
Hong-Ying SUN
1
;
Qiao XUE
;
Ying-Qiu PAN
;
Ding DING
;
Jing CHEN
;
Shu-Qing CHEN
Author Information
1. Institute of Pharmacology, Toxicology and Biochemistry, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Bacterial;
biosynthesis;
immunology;
Antibodies, Monoclonal;
biosynthesis;
immunology;
Antineoplastic Agents;
administration & dosage;
analysis;
immunology;
Enterotoxins;
administration & dosage;
analysis;
immunology;
Enzyme-Linked Immunosorbent Assay;
methods;
Hybridomas;
secretion;
Injections;
Mice;
Mice, Inbred BALB C;
Quality Control;
Rabbits;
Staphylococcus aureus;
chemistry
- From:
Acta Pharmaceutica Sinica
2008;43(8):801-805
- CountryChina
- Language:Chinese
-
Abstract:
The filtrate of Staphylococcus aureus culture has been used in an ampule form named as staphylococcal enterotoxin C injection for cancer therapy in clinic for ten years in China and proved to be effective. The active constituent of three kinds of injections is claimed to be staphylococcal enterotoxin C2 (SEC2), and the content of SEC2 is used as quality control. However, the correct content of SEC2 was not known and the relative amount of SEC2 was very low because of the complicated components of the filtrate. In this research, we established a proper ELISA system for the detection of SEC2 in staphylococcal enterotoxin C injection, which will improve the quality control of the injection. We produced and identified polyclonal and monoclonal antibodies of SEC2 and established BA-ELISA method based on the method of sandwich ELISA. It was found that the BA-ELISA method had good specificity, sensitivity and reproducibility, and being able to detect SEC2 at concentration from 2 to 20 ng x mL(-1), with an average CV value of 5.08%. The SEC2 content in staphylococcal enterotoxin C injection was calculated. There is some difference between the actual and labeled contents in the injections.
