Study on pan-resistant Klebsiella pneumoniae harboring blaKPC-2 type carbapenemase gene from a hospital outbreak in Huzhou, Zhejiang
10.3760/cma.j.issn.0254-6450.2010.05.019
- VernacularTitle:携带blaKPC-2型碳青霉烯酶基因泛耐药肺炎克雷伯菌医院内感染暴发的病原学分析
- Author:
Zhi-Mi HUANG
1
;
Jia-Rui MI
;
Yi-Quan SHENG
;
Yu-Xiu ZOU
;
Qiu-Ju CHU
;
Li-Wei GE
;
Hai-Yan YANG
Author Information
1. 解放军第98医院
- Keywords:
Klebsiella pneumoniae;
Pan-resistance;
Carbapenemases;
Drug resistance gene
- From:
Chinese Journal of Epidemiology
2010;31(5):559-562
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the status of genotype of the KPC(Klebsiella pneumoniae carbapenemase)-encoding genes in Pan-resistant K. Pneumoniae, isolated from the 98th Hospital of People' s Liberation Army, Huzhou district, Zhejiang province, China. Methods 19 strains of Pan-resistant K. Pneumoniae were isolated from the inpatients between November, 2008 and July,2009. Phenotypic confirmatory test for suspected carbapenemases production were carried out by Modified Hodge test. Carbapenemase gene of blaKPC was analyzed by PCR and verified by DNA sequencing. Results In 19 strains of K. Pneumoniae, the positive rates of Modified Hodge test and gene of blaKPC were both 100.0%. These genes all belonged to blaKPC-2 subtype confirmed by nucleotide sequence analysis. Among them, the blaKPC-2 gene sequence of the HZ001 strain (its original serial number was HZ9871 ) had been registered in GenBank (GenBank Accession Number: GU086225).Conclusion All of the Pan-resistant K. Pneumoniae isolated from the inpatients harbored blaKPC-2 type carbapenemases gene and causing an outbreak in a hospital. Carbapenemases that producing type KPC-2 might be the major reason which causing the resistance to Carbapenems antibiotics.
