Subtype and transmission of HIV strain in both HIV infected spouses in Zhejiang province
10.3760/cma.j.issn.0254-6450.2010.06.008
- VernacularTitle:浙江省46对夫妻双方感染艾滋病毒者中病毒株亚型及传播关系的研究
- Author:
Xiao-Hong PAN
1
;
Jia-Feng ZHANG
;
Ya-Ping YAO
;
Yun XU
;
Jie-Zhe YANG
;
Zhi-Hong GUO
Author Information
1. 浙江省疾病预防控制中心
- Keywords:
Human immunodeficiency virus;
Genetic subtype;
Transmission relationship;
Spouses
- From:
Chinese Journal of Epidemiology
2010;31(6):638-641
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse the subtype and transmission of HIV strain in both HIV infected spouses. Methods Reported both HIV infected spouses were selected as objects. Analysis on genetic sequence and high risk behaviors was carried out. Subgenomic gag was amplified by nest-PCR analysis of whole blood samples from objects. Genetic subtype characterization of HIV was identified and pairwise genetic distances were calculated. Sequence relationships were also examined by phylogenetic tree analysis. Results Genetic sequences of 46 pairs (92 cases) were obtained. The genetic subtype comprised 39 CRF01_AE(42.4%), 10 CRF07_BC(10.9%), 18 CRF08_BC (19.6%),18 B (19.6%) ,5 C (5.4%) and 2 CRF02_AG(2.2%). 44 pairs had the same subtype between the two partners, accounted for 95.7% of the total. 33 of the 41 pairs with phylogenetic tree analysis were found having epidemiological linkage in pair wise. Sexual behaviors of out-marriage were the main risk factors of CRF01_AE and CRF08_BC and CRF02_AG strains infection. Blood transmission was associated with B and CRF07_BC. Conclusion The HIV strains subtype detected in HIV infected spouses characterized with diversity. CRF01_AE was the main strain subtype. The main route of transmission to the spouses was through unprotected sexual contacts. Surveillance programs on HIV infected partner together with intervention between the spouses should be improved.
