Isolation and identification of a Banna virus strain from mosquitoes in Yunnan province

VernacularTitle:云南省一株版纳病毒的分离和鉴定
Author: Xiao-Hong SUN ¹ ; Xiao-Long ZHANG ; Yu-Fu LIU ; Jing WANG ; Lian WEI ; Xu GAO ; Wen ZHAO ; Xun ZHANG ; Xue-Bing YANG ; Le ZHANG
Author Information

1. 中周检验检疫科学研究院
Keywords: Banna virus; Sequence analysis
From: Chinese Journal of Epidemiology 2010;31(6):685-687
CountryChina
Language:Chinese
Abstract: Objective To isolate and identify Banna virus(BAV)from mosquitoes collected in Mengla county of Yunnan province.Methods Mosquito samples were collected in houses and stock yards in Mengla county,2008.Mosquitoes were homogenized and incubated onto both C6/36 and BHK21 cells.The new isolate was identified by using ELISA and RT-PCR.The sequences of segment 5.8 and 11 of BAV were amplified by RT-PCR and determined.Phylogenetic analysis on the new BAV were performed using MEGA4 program.Results 1731 mosquitoes representing 7 species were collected with one strain of BAV isolated and identified.Phylogenetic analysis based on sequences of segment 8 showed the new isolate was closed to BAV strain isolated in Yunnan,but segment 11 sequence was closed to Vietnam strain.Conclusion Results of phylogenetic analysis implied that the BAV re-assortment might have been occurred both in Chinese and Vietnam strains.