Ectopic Expression of Ephrin-A5 Under the EphA8 Promoter at the Anterior Region of the Superior Colliculus.
- Author:
Hyuna NOH
1
;
Soochul PARK
Author Information
- Publication Type:Original Article
- Keywords: ephrin-A5; EphA8; superior colliculus; topographic mapping
- MeSH: Animals; Axons; Chromosomes, Artificial, Bacterial; Ephrin-A5; Homologous Recombination; Humans; Mice; Mice, Transgenic; Retina; Superior Colliculi
- From:Experimental Neurobiology 2010;19(1):49-53
- CountryRepublic of Korea
- Language:English
- Abstract: EphA/ephrin-A mediated signaling has emerged as a key mechanism regulating axon guidance and topographic mapping, particularly in the well-characterized visual system from the retina to the superior colliculus (SC). In this study, EphA8 bacterial artificial chromosome (BAC) was manipulated to contain a floxed eGFP and human ephrin-A5 expression cassette using homologous recombination method. In the mice containing the recombinant BAC, it was shown that GFP is expressed in an anterior>posterior gradient in the SC. Furthermore, when these mice were crossed with the transgenic mice expressing Cre under the EphA8 promoter, it was evident that a GFP expression cassette was eliminated, and that human ephrin-A5 was ectopically expressed in the anterior region of the SC. This transgenic model would be useful to analyze the role of ephrin-A5 in the SC during the retinocollicular topography formation.
