Genotyping 238 HBV strains using type-specific primer PCR combined with type-specific nucleotide analysis.
- Author:
Ai-Zhong ZENG
1
;
Ai-Long HUANG
;
Jin-Jun GUO
;
Xiao-Yan DENG
;
Qing-Ling LI
;
Wen-Xiang HUANG
Author Information
- Publication Type:Journal Article
- MeSH: DNA Primers; DNA, Viral; blood; genetics; Female; Genotype; Hepatitis B virus; genetics; Hepatitis B, Chronic; virology; Humans; Male; Nucleotides; genetics; Polymerase Chain Reaction; methods; Sequence Analysis, DNA
- From: Chinese Journal of Hepatology 2008;16(2):84-87
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a set of suitable and reliable methods for HBV genotyping and to study the distribution of HBV genotypes.
METHODSType-specific nucleotides were searched through alignment of S genes (more than 1000 sequences) listed in GenBank. Then, type-specific primers were designed and type-specific primer PCR was used to genotype the 238 HBV strains. S genes of the untyped strains were further amplified and sequenced to find out their genotypes with type-specific nucleotide analysis.
RESULTSAll the 238 HBV strains were genotyped. 159 (66.8%) cases were genotype B, 69 (28.9%) were genotype C, 6 (2.5%) were mixtures of genotypes B and C and 4 (1.6%) were mixtures of genotypes B and D. No genotypes of A, E, F, G, and H were found.
CONCLUSIONGenotypes B and C are the most common types for HBV strains. Mixtures of genotypes B and C or genotypes B and D coinfection rarely existed. There is no relationship between the gender of the patients and HBV genotypes (X2 = 0.794, P more than 0.05).
