Effect of epigallocatechin-3-gallate on the invasiveness of hepatocarcinoma cells in vitro.
- Author:
Li LU
1
;
Hui-Min LIU
;
Wei-Xue TANG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; pathology; Catechin; analogs & derivatives; pharmacology; Cell Adhesion; Cell Survival; Hep G2 Cells; Humans; Liver Neoplasms; pathology; Matrix Metalloproteinase 2; metabolism; Matrix Metalloproteinase 9; metabolism; Mucin-1; metabolism; Neoplasm Invasiveness; Plant Extracts; pharmacology
- From: Chinese Journal of Hepatology 2007;15(11):825-827
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe effect of EGCG on the invasion of HepG2 cells in vitro was studied.
METHODSThe expressions of MUC1 in HepG2 cells before and after they were treated with EGCG were studied with immunohistochemistry. The effects of EGCG on the invasion of HepG2 cells were evaluated using Transwell chambers attached with polycarbonate filters and reconstituted basement membranes (Matrigel). Gelatin zymography was performed to detect the secretion of MMP-2 and MMP-9.
RESULTSEGCG reduced the expression of MUC1, suppressed significantly the invasion of tumor cells into the basement membranes (P < 0.01) and reduced the secretion of MMP-2, MMP-9.
CONCLUSIONEGCG has anti-invasion effects on HepG2 cells.
