Study on the induced differentiation of bone marrow-derived mesenchymal stem cells into inner ear hair cell-like cells in vitro.
- Author:
Yong-ming YANG
1
;
Hong-qun JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; Cell Differentiation; Cells, Cultured; Hair Cells, Auditory, Inner; cytology; Mesenchymal Stromal Cells; cytology; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2010;45(11):919-923
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the feasibility of orientation-induced differentiation of rat bone marrow-derived mesenchymal stem cells (MSC) into cochlear hair cell-like cells in vitro.
METHODSMSC were extracted, cultured and identified. The organ of Corti was isolated from post natal day 1-day 3 Sprague-Dawley rats. Then MSC were co-cultured with organ of Corti for 14 days in vitro. The expression of hair cell markers (myosin VIIa, math1 and calretinin) was detected by immunocytochemical analyses and by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTSThe differentiated cells were immunopositive for myosin VIIa, calretinin and math1. And RT-PCR results indicated that these differentiated cells expressed hair cell markers Math1 and myosin VIIa.
CONCLUSIONSMSC cultured in vitro could be successfully induced to differentiate into hair cell-like cells with hair cell molecular markers.
