Study on the induced differentiation of bone marrow-derived mesenchymal stem cells into inner ear hair cell-like cells in vitro

VernacularTitle:体外诱导大鼠骨髓间充质干细胞向内耳毛细胞样细胞分化的研究
Author: Yong-Ming YANG ¹ ; Hong-Qun JIANG
Author Information

1. 景德镇市第一人民医院
Keywords: Mesenchymal stem cells; Organ of Corti; Hair cells; Cell differentiation; Rats,Sprague-Dawley
From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2010;45(11):919-923
CountryChina
Language:Chinese
Abstract: Objective To investigate the feasibility of orientation-induced differentiation of rat bone marrow-derived mesenchymal stem cells (MSC) into cochlear hair cell-like cells in vitro. Methods MSC were extracted, cultured and identified. The organ of Corti was isolated from post natal day 1-day 3 SpragueDawley rats. Then MSC were co-cultured with organ of Corti for 14 days in vitro. The expression of hair cell markers (myosinⅦa, math1 and calretinin) was detected by immunocytochemical analyses and by reverse transcription-polymerase chain reaction (RT-PCR). Results The differentiated cells were immunopositive for myosinⅦa, calretinin and math1. And RT-PCR results indicated that these differentiated cells expressed hair cell markers Math1 and myosinⅦa. Conclusions MSC cultured in vitro could be successfully induced to differentiate into hair cell-like cells with hair cell molecular markers.