Expression of brain-derived neurotrophic factor modified bone marrow mesenchymal stem cells in the cochlea of drug deafened guinea pigs and its protection role
10.3760/cma.j.issn.1673-0860.2010.11.013
- VernacularTitle:脑源性神经营养因子基因修饰的骨髓间充质干细胞在药物致聋豚鼠内耳的表达及保护作用
- Author:
Guan-Gui CHEN
1
;
Ding-Hua XIE
;
Qian-Xu LIU
;
Zhi-Qiang TAN
Author Information
1. 广州医学院附属第二医院
- Keywords:
Brain-derived neurotrophic factor;
Mesenchymal stem cells;
Spiral ganglion;
Hearing loss,sensorineural;
Mesenchymal stem cell transplantation;
Apoptosis
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2010;45(11):924-929
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of brain-derived neurotrophic factor (BDNF)gene modified bone marrow mesenchymal stem cells (MSC) in the cochlea of drug-deafened guinea pigs and its protection to spiral ganglion cells (SGC). Methods Guinea pigs deafened by subcutaneous injection of amikacin were randomly divided into two groups, BDNF gene modified bone marrow MSC were injected into the cochlea through fenestration of scala tympani in the experimental group, while artificial perilymphatic fluid were injected in the control group. Experimental animals were executed at 7 and 28 days postoperation. Expression of BDNF mRNA was examined by quantitate real time RT-PCR, histological images of cochlear sections were analyzed to calculate the cellular density of the SGC, and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) was used to identify the apoptotic neurons. Results The BDNF expressive level in experimental group was higher than in the control group at 7 d and 28 d postoperation, whose differences were both statistically significant (P ＜ 0.01 ). And, It showed a higher aboundance of ganglion cell numbers, as well as a decreased apoptotic index in experimental group compared with the control group at 7 d and 28 d post-operation, whose differences were all statistically significant( P ＜0. 01 ). Conclusion BDNF gene modified MSC could maintain expression for at least 28 days after transplantation into cochlea of drug deafened guinea pigs, and protect SGC.
