Influence of celecoxib on invasiveness of human high-metastatic nasopharyngeal carcinoma cell line CNE-2Z
10.3760/cma.j.issn.1673-0860.2010.11.016
- VernacularTitle:塞来昔布对人高转移性鼻咽癌CNE-2Z细胞侵袭力的影响
- Author:
Wei-Ren LUO
1
;
Li-Xia LI
;
Si-Yi LI
;
Han-Guo JIANG
;
Xiao-Yi CHEN
Author Information
1. 广东医学院
- Keywords:
Sulfonamides;
Pyrazoles;
Cadherins;
Nasopharyngeal neoplasms;
Carcinoma,squamous cell;
Tumor cells,cultured;
Neoplasm invasiveness
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2010;45(11):941-945
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism (a selective cyclooxygenase-2 inhibitor) on invasive ability of human nasopharyngeal carcinoma (NPC) line CNE-2Z. Methods The proliferation of NPC cells was examined by MTT assay. The invasive and migrating ability of NPC cells was detected with transwell chamber. E-cadherin protein expression was detected by immunocytochemistry and the expressions of Cox-2 and E-cadherin mRNA were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). Results MTT showed that celecoxib inhibited CNE-2Z proliferation in dose-dependent manner, the survival rate of cells treated with 25, 50, 100 μ mol/L celecoxib (-x±s) for 24 h was(94. 75 ±1.34)%, (91.77 ±2. 70)% , (64. 54 ± 1.20)%, respectively, and the survival rate of cells treated for 48 h was ( 88.41±1.28 ) %, ( 78. 84 ± 1.56 ) %, ( 52. 46 ± 2. 25 ) %, respectively, the concentration of 50% inhibition concentration of a substance (IC50) was 100 μmol/L, the difference was statistically significant between different concentration groups in the same time-point ( repectively, F were 462. 204 and 1328. 306, P ＜0. 01 ). Treated with different concentrations of celecoxib(0, 25, 50 μmol/L) for 24, the cell numbers (-x±s) through PVPF by tumor invasion assay were (263.7 ± 13.5), (185.3 ±8.7) and (144. 0 ± 8. 2), the difference was statistically significant between the experimental and control group (F =102. 089, P ＜0. 01 ). Immunocytochemistry showed that celecoxib significantly induced the increase of Ecadherin protein expression, also with a dose-dependence in 0 μmol/L, 25 μmol/L, 50 μmol/L group was (21.7 ±2. 6), (28. 7 ±2. 4), (40. 3 ± 1.3), and 50 μmol/L group increased significantly ( F =78. 637,P ＜0. 01 ). RT-PCR showed that celecoxib reduced the expression of Cox-2 mRNA expression in 25, 50 μmol/L group decreased significantly compared with the control group (respectively, t were 23. 950 and 36. 651, P ＜ 0. 01 ), but it enhanced the expression of E-cadherin mRNA expression in 25, 50 μmol/L group was significantly higher ( respectively, t were 35. 829 and 81. 497, P ＜0. 01 ). Conclusion Celecoxib can inhibits the invasive ability of NPC cell line CNE-2Z, which possibly relates with the upregulated expression of E-cadherin.
