Relationship between allergic symptoms and RORC2 and IL-17 in patients with allergic rhinitis
10.3760/cma.j.issn.1673-0860.2011.02.012
- VernacularTitle:转录因子RORC2和白细胞介素17的表达与变应性鼻炎症状关系的研究
- Author:
Han-Gui LU
1
;
Hua PENG
;
De-Hua CHEN
;
Tian-Ying LI
;
Jian-Bo SHI
Author Information
1. 汕头市中心医院
- Keywords:
Rhinitis,allergic,perennial;
Interleukin 17;
RORC2 transcription factor
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2011;46(2):144-148
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the relationship between allergic symptoms and retinoic acid related orphan receptor variant 2 (RORC2) and interleukin(IL) 17 in patients with allergic rhinitis(AR).Methods Blood sample,nasal secretion and nasal mucosa were taken from 23 patients with AR and 16 health individuals.The expression of RORC2 and IL-17 were detected by immunohistochemistry and quantitative real-time fluorescence reverse polymerase chain reaction.The allergic symptoms in patients were graded.Results The rate of positive cells of RORC2 and IL-17 in AR group were 0.17 ±0.05 and 0.72 ±0.13,higher than the 0.05 ± 0.02 and 0.27 ± 0.11 of health controls,the difference was statistically significant(t were 9.51 and 11.92 respectively,all P ＜0.05＝.The expression level of RORC2 mRNA in nasal mucosa and peripheral blood of AR group were 0.063 ± 0.011 and 0.452 ± 0.031,higher than the 0.029 ±0.009 and 0.239 ±0.027 of health controls,the difference was statistically significant (t were 6.51and 3.35 respectively,all P ＜0.05＝.The concentrations of IL-17 in the nasal mucosa,nasal secretions and serum levels of AR group were (70.28 ± 10.69) ,(45.32 ± 8.55) and (6.76 ± 1.18) pg/ml,compared with (18.43 ± 8.34),(6.83 ± 1.31) and (0.74 ±0.05) pg/ml of controls,the difference was statistically significant(t were 7.92,17.66 and 15.43 respectively,all P ＜ 0.05＝.The allergy symptom scores of AR group were 9.43 ± 1.27.There were correlations between the allergic symptom and the expression of RORC2 mRNA and IL-17 in nasal mucosa and peripheral blood (r value were 0.820,0.746,0.629,0.841 respectively,all P ＜ 0.05＝.Conclusion RORC2 and IL-17 involved in the inflammatory response of AR and can be used as an indicator to judge the severity.
